Date Approved
2017
Degree Type
Open Access Senior Honors Thesis
Department or School
Chemistry
First Advisor
Dr. Heather Holmes
Second Advisor
Dr. Hedeel Evans
Abstract
Feline Leukemia Virus (FeLV) is a retrovirus that affects domesticated cats and other species in the Felis genus. There are three major subtypes, each characterized by unique disease progression and proviral sequences. FeLV-A is weakly pathogenic, -B has been associated with tumors, and -C causes severe anemia. Proviral sequences were amplified using PCR with FeL V-specific and adapter-specific primers. The method of primer walking has yielded consensus sequences for several fragments of 1,000-3,000 base pairs of proviral DNA. Sequences for ten different clones of the provirus envelope region from a FeL V positive blood sample were compared to known sequences and revealed novel insertions.
Recommended Citation
Sohl, Brianna, "Analysis of variable regions in feline leukemia virus envelope sequences" (2017). Senior Honors Theses and Projects. 536.
https://commons.emich.edu/honors/536