Author

Brianna Sohl

Date Approved

2017

Degree Type

Open Access Senior Honors Thesis

Department

Chemistry

First Advisor

Dr. Heather Holmes

Second Advisor

Dr. Hedeel Evans

Abstract

Feline Leukemia Virus (FeLV) is a retrovirus that affects domesticated cats and other species in the Felis genus. There are three major subtypes, each characterized by unique disease progression and proviral sequences. FeLV-A is weakly pathogenic, -B has been associated with tumors, and -C causes severe anemia. Proviral sequences were amplified using PCR with FeL V-specific and adapter-specific primers. The method of primer walking has yielded consensus sequences for several fragments of 1,000-3,000 base pairs of proviral DNA. Sequences for ten different clones of the provirus envelope region from a FeL V positive blood sample were compared to known sequences and revealed novel insertions.

Included in

Chemistry Commons

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