Date Approved

8-31-2012

Date Posted

4-25-2012

Degree Type

Open Access Thesis

Degree Name

Master of Science (MS)

Department

Health Sciences

Committee Member

SivaKumar Vallabhapurapu, PhD

Committee Member

Stephen Sonstein, PhD

Committee Member

Irwin Martin, PhD

Abstract

Genomic DNA was purified from Multiple Myeloma Cell Line JJN3, and BMF promoter region was PCR amplified and cloned into pCR 2.1 TOPO vector using One Shot® Chemically Competent E. coli. After confirmation of the clone through polymerase chain reaction (PCR) and restriction analysis, BMF promoter (2.5 Kb) was released from TOPO vector and subcloned into pGL3 promoter vector. The resulting pGL3-BMF-promoter vector was further amplified for MaxiPrep preparation. This plasmid can be used to study the regulation of BMF promoter by various transcription factors including NF-κB family members.

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