Date Approved
5-2013
Date Posted
4-4-2014
Degree Type
Open Access Thesis
Degree Name
Master of Science (MS)
Department or School
Chemistry
Committee Member
Steven Pernecky, Ph.D., Chair
Committee Member
Heather Holmes, Ph.D.
Committee Member
Cory Emal, Ph.D.
Abstract
Myoglobin (Mb) and Myoglobin-H (Mb-H) react with glycerophosphocholine (GPC) lipid in the presence of oxygen. This reaction allows the heme group in the protein the chance to induce peroxidation reactions with the lipid. Several aldehyde products of this type of reaction with arachadonic acid have previously been determined, with Mb generating greater quantities of aldehyde products than Mb-H. The focus of this research was to establish the presence of some products from the reactions of Mb and Mb-H with GPC (which contains arachadonic acid) that are more prevalent from Mb-H than Mb, or that only form from the reaction of Mb-H with GPC. A combination of spectrophotometry, TLC, and ion-trapping GC-MS was used to establish the generation of an oxidation product of arachidonic acid metabolism that is generated in larger quantities by the reaction of Mb-H with GPC than by the reaction of Mb with the same lipid.
Recommended Citation
Lawrence, Kathryn Rae, "Arachidonate oxidation metabolite profiles for myoglobin and a structurally altered form of myoglobin produced in muscle disease or trauma" (2013). Master's Theses and Doctoral Dissertations. 543.
https://commons.emich.edu/theses/543