Open Access Senior Honors Thesis
Steven Pernecky, PhD
Lynne Shetron-Rama, PhD
Interest in determining the mechanism by which pro biotic bacteria in the gastrointestinal tract produce short chain fatty acids (SCFA) has increased over the past few years. This study uses gas chromatography and mass spectrometry to characterize lactate production in aerobic test tube cultures, aerobic bioreactor cultures, and anaerobic bioreactor cultures. To collect anaerobic bioreactor samples, a novel in vitro anaerobic model was developed. The pH and colony forming units of each bacterial sample was also measured in order to understand the correlation between lactate production and bacterial growth. The purpose of this study was to analyze production of SCFA in co-cultures and to develop an anaerobic model to analyze SCFA production by anaerobic bacteria. 4
Rautiola, Elyssa, "Short Chain Fatty Acid Production by Probiotic Organisms in the Gastrointestinal Tract" (2013). Senior Honors Theses & Projects. 328.