Open Access Senior Honors Thesis
Use of I-pyrenyldiazomethane (PDAM) for derivatizing short-chain fatty acids (SCFA) is a successful way to analyze complex SCF A samples quickly and with minimal sample preparation. The costs associated with ordering PDAM from a commercial source can be high, typically more than $300 not including shipping costs. This project developed a simple two-step synthesis procedure that can be used in undergraduate chemistry laboratories. We found that the PDAM produced by this procedure is pure enough to use for our derivatization needs without further purification. This synthesis can be used to further explore the use of PDAM for fast and simple analysis of SCF A without excessive material costs.
Kalvaitis, Mary E., "Synthesis of 1-Pyrenyldiazomethane for Derivatization of Short-Chain Fatty Acids" (2013). Senior Honors Theses & Projects. 341.