Open Access Thesis
Master of Science (MS)
Robert S. Winning, PhD
Glenn K. Walker, PhD
Tamara L. Greco, PhD
Eph receptors and their ligands, ephrins, have been implicated in many important developmental processes and in tumorigenesis. The activation of EphA4 receptors in the frog, Xenopus laevis, causes loss of cell adhesion, tight junctions, and stress fibers. Previous studies revealed that these receptors achieve their cellular effects at least in part by inhibiting RhoA GTPase. However, what links these GTPases with the EphA4 receptor remained unknown.
In an attempt to investigate involvement of the Src-like tyrosine kinase p59fyn in EphA4 signaling, a series of experiments was planned using the chimeric receptor EPP. The findings presented in this thesis indicate that the Srclike tyrosine kinase, p59fyn, acts as a functional link between the EphA4 receptor and RhoA GTPase in Xenopus laevis embryonic cells. Moreover, an activated EphA4 receptor achieves its cellular effects through activation of p59fyn, which in turn inhibits RhoA.
Grabauskiene, Svetlana, "Activated EphA4 receptor in Xenopus laevis acts rhrough src- like tyrosine kinase p59fyn to inhibit RhoA GTPase" (2003). Master's Theses and Doctoral Dissertations. 71.