Quantification of autophagosome size and formation rate by electron and fluorescence microscopy in baker’s yeast

Authors

• Steven K. Backues, Eastern Michigan University
• Daniel J. Klionsky, University of Michigan - Ann Arbor

Document Type

Book Chapter

Publication Date

2022

Department/School

Biology

Publication Title

Imaging and Quantifying Neuronal Autophagy

Abstract

The use of both transmission electron microscopy and fluorescence microscopy have provided tremendous advances to our understanding of autophagosome formation in baker’s yeast, Saccharomyces cerevisiae. In the last decade, parallel techniques have been developed for both types of microscopy that allow the quantification of the rate of autophagosome formation. Importantly, these techniques, unlike other measures of total autophagic flux, allow a researcher to distinguish between effects on autophagosome size and autophagosome number. This has led to the discovery that certain autophagy proteins (e.g., Atg8) contribute primarily to the control of autophagosome size, whereas others (e.g., Atg9) are principally involved in controlling autophagosome number, suggesting different roles for these proteins in the autophagosome formation process.

Comments

S. K. Backues is a faculty member in EMU's Department of Chemistry.

Recommended Citation

Backues, S. K., & Klionsky, D. J. (2022). Quantification of autophagosome size and formation rate by electron and fluorescence microscopy in baker’s yeast. In B. Loos & E. Wong (Eds.), Imaging and quantifying neuronal autophagy (Vol. 171). Humana. https://doi.org/10.1007/978-1-0716-1589-8_1