Date Approved
2023
Date Posted
2023
Degree Type
Open Access Senior Honors Thesis
Department or School
Chemistry
First Advisor
Steven Backues, Ph.D.
Second Advisor
Hedeel Evans, Ph.D.
Abstract
Autophagy is a necessary recycling process that occurs in cells, but the functions of the proteins that carry this out are not completely understood. The protein ATG10 has a distinct role in forming autophagosomes, large double membrane vesicles necessary for this process. ATG10 forms a covalent bond between the proteins ATG12 and ATG5 but its final effect on autophagosome formation is still unclear. We are using DNA manipulation techniques to add a myc epitope tag to yeast ATG5 which will allow us to measure the effect of atg10 mutants on ATG12-5 conjugation via Western Blot. These results will help us correlate ATG10 activity to autophagosome size and number.
Recommended Citation
Campbell, Sophie, "Tagging ATG5 with an epitope tag to measure the amount of ATG12-5 conjugation in atg10 mutants" (2023). Senior Honors Theses and Projects. 791.
https://commons.emich.edu/honors/791
