Date Approved
2021
Degree Type
Open Access Thesis
Degree Name
Master of Science (MS)
Department or School
Biology
Committee Member
Hannah Seidel, PhD
Committee Member
Aaron Liepman, PhD
Committee Member
Robert Winning, PhD
Abstract
The abbreviated cell cycle is a poorly understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples that were easier to find and focus on at the microscope. These optimizations, combined with future labeling of DNA replication, will expand our understanding of how cells with an abbreviated cell cycle maintain genome stability.
Recommended Citation
Campbell, Taylor, "Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in Caenorhabditis elegans (roundworm) germline stem cells" (2021). Master's Theses and Doctoral Dissertations. 1095.
https://commons.emich.edu/theses/1095