Date Approved
2007
Degree Type
Open Access Thesis
Degree Name
Master of Science (MS)
Department or School
Biology
Committee Member
Dr. Marianne Laporte, Chair
Committee Member
Dr. Steven Pernecky, Member
Committee Member
Dr. Glenn Walker, Member
Abstract
Stomata are leaf structures critical for plant survival because they modulate the rate of transpiration. This study will examine a possible mode for regulating stomatal density by over-expressing NADP-dependent malic enzyme (NADP-ME), which is thought to deplete the counter-anion pool in Arabidopsis thaliana guard cells. It was hypothesized that over-expressing NADP-ME in Arabidopsis will elevate stomatal density. Scanning electron microscopy was employed to determine if any differences exist in numbers of stomatal complexes among transgenic lines and wild-type Arabidopsis. The results indicated a significant increase in stomatal numbers among some transgenic plant lines relative to the wild-type. Second, a series of molecular tests were utilized to provide NADP-ME activity and transgene expression evidence. These included western blots, tissue assay blots, enzyme activity assays, and RT-PCR reactions. Altering NADP-ME expression levels may present a putative approach for influencing stomatal development.
Recommended Citation
Gabor, Michael M., "A putative approach for influencing stomatal density by over-expressing NADP-dependent malic enzyme in arabidopsis" (2007). Master's Theses and Doctoral Dissertations. 42.
https://commons.emich.edu/theses/42
Comments
Dr. Robert Winning, Thesis Committee Member