Date Approved
2018
Degree Type
Open Access Thesis
Degree Name
Master of Science (MS)
Department or School
Psychology
Committee Member
Joseph Breza, Ph.D.
Committee Member
Thomas Waltz, Ph.D., Ph.D.
Committee Member
Kenneth Rusiniak, Ph.D.
Abstract
This thesis describes a series of experiments designed to evaluate the hypothesis that Type I taste receptor cells play a critical role in the detection and transduction of sodium taste via of epithelial sodium channels (ENaCs). Experiment 1 validated the function of a simple and affordable behavioral apparatus (hardware and software) for testing taste preference and taste aversion in mice. Experiment 2 demonstrated a pharmacological method for rapid induction of salt appetite in mice. Experiment 3 showed that optogenetic stimulation of Type I taste receptor cells (TRCs) in transgenic mice could drive consumption of tap water under conditions of salt hunger. The fourth and final experiment assessed whether conditioned taste aversions to sodium would generalize to optogenetic stimulation of Type I taste receptor cells in transgenic mice, with inconclusive results.
Recommended Citation
Raymond, Martin, "Optogenetic examination of salt taste in mice" (2018). Master's Theses and Doctoral Dissertations. 928.
https://commons.emich.edu/theses/928